The actuality is that the biology of GVHD is likely much more complex Your browser does not support SVG
PBMCS from two donors, irradiate one fraction and label other Mix together Your browser does not support SVG
PBMCS from two donors, irradiate one fraction and label other Mix together Allow alloactivation of T cells to proceed over the following 3-5 days Your browser does not support SVG
PBMCS from two donors, irradiate one fraction and label other Mix together Allow alloactivation of T cells to proceed over the following 3-5 days Dividing T cells will dilute out the green label (CFSE) and can be quantified flow cytometrically Your browser does not support SVG
How can single cell RNA seq performed on cells from an MLR inform us of the transcriptional events that define alloproliferation
Three donors were utilized (A, B, C) MLR was set up using each donor as a responder on each other donor (e.g A on B, A on C, B on A, B on C) Controls included T cells cultured with anti-CD3/anti-CD28 microbeads Cells were harvested and sorted by CFSE dilution (e.g. Division 0, Division 1, … Division 4)
